Western blot analysis of GEM in extracts of various cell lines. Samples were incubated with GEM Polyclonal antibody (Product # PA5-109761) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Western blot analysis of GEM in HeLa, 293T. Samples were incubated in polyclonal GEM antibody (Product # PA5-109761) using a dilution of 1:1000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10000.
Supplier Page from Thermo Fisher Scientific for GEM Antibody